Thiamine (Vitamin B1)

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Price of the test:

$83 per analysis

Limit of Detection:

0.02 mg/100g to 0.1 mg/100g (depending on sample weight)

Sample Size Requirements:

10 grams for dry samples
20 grams for liquid samples


Auto-analyzer with fluorescence detection

Reportable Units:

mg/100 grams

Turn Around Time:

10 Business Days

Is Rush Available?


Method Reference:

AOAC 942.23, 970.65 and 981.15

Method Description:

Samples are extracted by autoclaving in HCl to breakdown the matrix. A buffer and enzyme are added to convert any thiamin phosphate to thiamin. Alkaline potassium ferricyanide is added to the filtrates to oxidize and convert thiamin to thiochrome. The thiochrome is extracted into iso-butyl alcohol and measured fluorometrically. Blanks are determined by adding NaOH instead of ferricyanide. The thiamin level is determined by comparing the net fluorescence to a standard curve.

Information Required by Submitter:

Please supply an estimate of expected thiamine content.

Please indicate the presence of high levels of iron.

Samples of "Vitamin Concentrates" should be clearly labeled.

Additional Information:

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.

High iron content may interfere with the reaction chemistry. Vitamin concentrate results typically have higher-than-normal method variability.