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Dietary Fiber, Total (Gravimetric)

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TECHNICAL DATA SHEET

Price of the test:

$172 per analysis

Acceptable Matrices:

All foods and food products


Limit of Detection:

0.1%


Sample Size Requirements:

40 grams


Equipment:

Standard Gravimetric Analytical Chemistry Equipment


Reportable Units:

Percent by weight of sample as received


Turn Around Time:

10 Business Days


Is Rush Available?

Yes


Method Reference:

AOAC 991.43 with modifications


Method Description:

Duplicate samples (defatted if necessary) are digested with three enzymes (alpha-amylase, protease and amyloglucosidase) to remove starch and protein. Ethanol is added to precipitate soluble dietary fiber. The resulting solution is filtered and the residue is dried and weighed. Protein is determined on the residue of one of the duplicates; ash on the other. Total dietary fiber (TDF) is calculated as the percent residue minus protein and ash.


Information Required by Submitter:

Please supply expected estimates. Indicate matrix type and indicate high fat (>5%) and high protein (>20%) samples. Indicate whether the sample contains fructans (inulin or fructooligosaccharides (FOS)), or psyllium, flax and gum (i.e. guar gum).


Additional Information:

This test is ISO/IEC 17025 accredited through A2LA, Certificate #2769.01.

Dietary Fiber is the portion of the carbohydrates which is not digested in the human gut. It is composed of both soluble and insoluble components. These methods are applicable to all food products, however samples containing psyllium or fructans should be submitted under specific test codes. High-fat (>5%) and high-protein (>20%, depending on matrix) samples require special handling.