Back

Resistant Oligosaccharides with Total, Soluble, and Insoluble Dietary Fiber

PDFdnwIcon Testing Service Catalog (PDF) Print Email

TECHNICAL DATA SHEET

Price of the test:

$440 per analysis

Acceptable Matrices:

All foods and food products


Limit of Detection:

Total Dietary Fiber - 0.1%
Soluble Fiber – 0.1%
Insoluble Fiber – 0.1%
Resistant Oligosaccharides - 0.4% (w/w)


Sample Size Requirements:

40 grams


Equipment:

General Gravimetric Analytical Chemistry Equipment
HPLC-Refractive Index Detector


Reportable Units:

Percent by weight of sample as received


Turn Around Time:

15 Business Days


Is Rush Available?

No


Method Reference:

AOAC 2001.03 and AOAC 991.43


Method Description:

This method determines traditional total dietary fiber (TDF), soluble dietary fiber (SDF), insoluble dietary fiber (IDF), and digestion-resistant oligosaccharides (ROs) in both food ingredients and food products. Samples (defatted if necessary) are first run by a traditional dietary fiber method, in which digestion with three enzymes (protease, amylase, and amyloglucosidase) is carried out in a phosphate buffer. Any solid particles not in solution after digestion are considered insoluble dietary fiber and are filtered off and measured gravimetrically. The filtrate from this first filtration is collected and ethanol is added to precipitate the soluble dietary fiber. The soluble fraction is then filtered and measured gravimetrically. The final filtrate solution from the soluble dietary fiber analysis is collected after filtering off the precipitated soluble fiber fraction. Resistant oligosaccharides are soluble in this alcoholic solution, so in order to quantitate them, the filtrate is evaporated to 20 mL. Deionized through a series of anion and cation exchange columns to remove interferences from buffers, salts, amino acids, peptides, and protein and then reconcentrated to 10 mL. The solution is mixed well and syringe filtered prior to analysis by size exclusion HPLC. The size exclusion HPLC columns separate sample solution components into peaks based on molecular weight. The peaks corresponding to oligosaccharides larger than DP2 are quantitated, summed, and reported as total resistant oligosaccharides.


Information Required by Submitter:

Please supply expected estimates. Indicate matrix type and indicate high fat (>5%) and high protein (>20%) samples. Indicate whether the sample contains fructans (inulin or fructo-oligosaccharides (FOS)), or psyllium, flax and gum (i.e. guar gum).


Additional Information:

Resistant oligosaccharides are polysaccharides from DP3 and higher which are soluble in 78% ethanol and are resistant to digestion by amylase under the conditions of the traditional dietary fiber analysis (AOAC 991.43). In the past, this analysis has gone by names such as “fibersol” and “resistant maltodextrins.” At Medallion Labs, we feel that these terms are limiting because we know that other polysaccharides, such as polyfructans are also included in this result. We are using AOAC method 2001.03, but have renamed the result “resistant oligosaccharides” because we feel this is the proper, general classification for the molecules that are being measured.