Back

Nitrogen

PDFdnwIcon Testing Service Catalog (PDF) Print Email

TECHNICAL DATA SHEET

Price of the test:

$26 per analysis

Acceptable Matrices:

This method is universal for the determination of nitrogen content and can be used for nearly all food products.

Unacceptable Matrices:

Pure oils and shortenings cause fires in the instrument and almost always must be assayed by the Kjeldahl method.


Limit of Detection:

0.10%


Sample Size Requirements:

Please provide a sample of at least 20g to ensure results representative of sample submitted.


Equipment:

Protein/Nitrogen by combustion analyzer.


Reportable Units:

% Nitrogen


Turn Around Time:

10 Business Days


Is Rush Available?

Yes


Method Reference:

AACC 46-30; AOAC 992.15


Method Description:

Total nitrogen is determined using a combustion instrument operating on the Dumas principle. This method is universal for the determination of nitrogen content and can be used for nearly all food products. Pure oils and shortenings may cause fires in the instrument and should always must be assayed by the Kjeldahl method.

The sample is completely combusted at high temperature in the presence of pure O2 (99.9%). Combustion products are H2O, CO2, NOx, and N2. Water vapor, oxygen and CO2 are removed from the resulting gas stream by a series of thermoelectric coolers and chemical sorbents. NOx is reduced to N2 when passed through a hot column that packed with copper. The resulting N2 is passed to a thermal conductivity detector where it is quantified (helium is the carrier gas).