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Sugar Profile by HPLC Expanded I (Raffinose and Stachyose)

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TECHNICAL DATA SHEET

Price of the test:

$115 per analysis

Acceptable Matrices:

Samples containing legumes and grains

Unacceptable Matrices:

Samples with high (>5%) salt content


Limit of Detection:

The detection level is 0.1%, with salt (NaCl) content < 5%


Sample Size Requirements:

20 grams


Equipment:

HPLC RI


Reportable Units:

% by weight


Turn Around Time:

10 Business Days


Is Rush Available?

Yes


Method Reference:

AOAC 977.20 with modifications

High Pressure Liquid Chromatographic Determination of Carbohydrates in Food Products: Evaluation of Method, J.W. DeVries, J.C. Heroff, and D.C.Egberg, J. Assoc. Off. Anal. Chem. 62 p1292 (1979)


Method Description:

This method directly determines the amounts of: fructose, glucose, sucrose, maltose, lactose, stachyose, and raffinose. The sugars are extracted and after the appropriate dilution and clean up are quantitated using High Performance Liquid Chromatography (HPLC).


Information Required by Submitter:

Please supply an estimate of the sugar level if possible.


Additional Information:

Stachyose and raffinose are trisaccharide and tetrasacchride sugars that are present in grains and legumes. This test is run as an addition to our Sugar Profile by HPLC.

The extract obtained for our normal sugar analysis is injected on an HPLC system employing a different mobile-phase. Other assay characteristics are the same as for Sugar Analysis.

This methodology is specific and all calculations are based on the response factors of known standards.

High (>5%) salt (NaCl) content may interfere with the analysis.