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Sugar Profile by HPLC Expanded II (Galactose)

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TECHNICAL DATA SHEET

Price of the test:

$104 per analysis

Acceptable Matrices:

Samples considered to be dairy products

Unacceptable Matrices:

Samples with high >5% salt content


Limit of Detection:

The detection level is 0.1%, with salt (NaCl) content < 5%


Sample Size Requirements:

20 grams


Equipment:

HPLC-RI


Reportable Units:

% by weight


Turn Around Time:

10 Business Days


Is Rush Available?

Yes


Method Reference:

AOAC 977.20 with modifications

High Pressure Liquid Chromatographic Determination of Carbohydrates in Food Products: Evaluation of Method, J.W. DeVries, J.C. Heroff, and D.C.Egberg, J. Assoc. Off. Anal. Chem. 62 p1292 (1979)


Method Description:

This method directly determines the amounts of: fructose, glucose, galactose, sucrose, maltose, and lactose. The sugars are extracted and after the appropriate dilution are quantitated using High Performance Liquid Chromatography (HPLC).


Information Required by Submitter:

Please supply an estimate of the sugar level if possible.


Additional Information:

Galactose is a monosaccharide that in combination with glucose forms lactose.

Samples are extracted in to water and diluted with acetonitrile. The extract obtained is injected onto an HPLC system, and the quantity of galactose is calculated by comparison with HPLC traces of injected standards of galactose. Other assay characteristics are the same as for the sugar analysis.

This methodology is extremely specific and all calculations are based on the response factors of known standards.

High (>5%) salt (NaCl) content may interfere with the analysis.