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Vitamin C

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TECHNICAL DATA SHEET

Price of the test:

$61 per analysis


Limit of Detection:

0.3 mg/100g to 2 mg/100g (depending on sample weight)


Sample Size Requirements:

10 grams for dry samples
20 grams for liquid samples


Equipment:

Fluorescence Spectrophotometer, Auto-analyzer with fluorescence detection


Reportable Units:

mg/100 grams


Turn Around Time:

10 Business Days


Is Rush Available?

Yes


Method Reference:

AOAC 967.22 with modifications and 984.26


Method Description:

Ascorbic acid is extracted into a methanol/metaphosphoric acid solution. The ascorbic acid is converted to dehydroascorbic acid, which is then reacted with ortho-phenylenediamine to form a fluorophor whose intensity is proportional to vitamin C concentration. A blank is simultaneously run on each sample to determine the level of background fluorescence. The total vitamin C content can then be calculated by comparing the net fluorescence to a standard curve.


Information Required by Submitter:

Please supply an estimate of expected vitamin C content. Notify Medallion Labs if the vitamin C source in your sample is encapsulated. Samples of "Vitamin Concentrates" should be clearly labeled.


Additional Information:

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.

Vitamin concentrate results typically have higher-than-normal method variability.