Vitamin C

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Price of the test:

$61 per analysis

Limit of Detection:

0.3 mg/100g to 2 mg/100g (depending on sample weight)

Sample Size Requirements:

10 grams for dry samples
20 grams for liquid samples


Fluorescence Spectrophotometer, Auto-analyzer with fluorescence detection

Reportable Units:

mg/100 grams

Turn Around Time:

10 Business Days

Is Rush Available?


Method Reference:

AOAC 967.22 with modifications and 984.26

Method Description:

Ascorbic acid is extracted into a methanol/metaphosphoric acid solution. The ascorbic acid is converted to dehydroascorbic acid, which is then reacted with ortho-phenylenediamine to form a fluorophor whose intensity is proportional to vitamin C concentration. A blank is simultaneously run on each sample to determine the level of background fluorescence. The total vitamin C content can then be calculated by comparing the net fluorescence to a standard curve.

Information Required by Submitter:

Please supply an estimate of expected vitamin C content. Notify Medallion Labs if the vitamin C source in your sample is encapsulated. Samples of "Vitamin Concentrates" should be clearly labeled.

Additional Information:

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.

Vitamin concentrate results typically have higher-than-normal method variability.