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Lipoxygenase

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TECHNICAL DATA SHEET

Price of the test:

Call for Pricing

Acceptable Matrices:

cereal grains, doughs, pastas, and vegetables


Limit of Detection:

0.01 units/gram


Sample Size Requirements:

20 grams


Equipment:

UV/Vis spectrophotometer, homogenizer, microplate reader


Reportable Units:

units per gram. 
One unit is defined as the change in absorbance at 234nm per minute from the hydroperoxidation of the cis, cis-1,4 pentadiene containing fatty acid. 
One unit is equivalent to the oxidation of 0.12 micromoles of linoleic acid.


Turn Around Time:

15 business days


Is Rush Available?

Yes


Method Reference:

Axelrod B, Cheesbrough TM, and Laakso S (1981) Lipoxygenase from soybeans, In "Methods in Enzymology", Vol. 71. (Ed.) JM Lowenstein.  Academic Press, New York

Ben-Aziz A, Grossman S, Ascarelli I, and Budowski P (1970) Linoleate oxidation induced by lipoxygenase and heme proteins: a direct spectrophotometric assay.  Anal. Biochem. 34:8


Method Description:

Samples are homogenized and enzymes are extracted into a cold, pH 6.5 buffer.  A protein determination by the Method of Bradford is performed on the enzyme extract (supernatant) to normalize subsequent enzyme results to the efficiency of the protein extraction.  The enzyme extract is mixed with linoleic acid or arachidonic acid.  Lipoxygenase activity is measured spectrophotometrically by the rate of formation of the fatty acid hydroperoxide at 234nm.


Information Required by Submitter:

Estimates of activity if available.
Composition of sample matrix.


Additional Information:

Please keep and ship samples frozen if possible.