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Polyphenol Oxidase

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TECHNICAL DATA SHEET

Price of the test:

Call for Pricing

Acceptable Matrices:

cereal grains, doughs, pastas, and vegetables


Limit of Detection:

0.01 units/gram


Sample Size Requirements:

20 grams


Equipment:

UV/Vis spectrophotometer, homogenizer, microplate reader


Reportable Units:

units per gram
One unit is defined as the change in absorbance at 410nm per minute from the formation of 1.2 benzoquinone


Turn Around Time:

15 business days


Is Rush Available?

Yes


Method Reference:

Anderson JV and Morris CF (2001) Crop Science 41:1697-1705.;AACC 22-85


Method Description:

Samples are homogenized and enzymes are extracted into a cold, pH 6.5 buffer.  A protein determination by the Method of Bradford is performed on the enzyme extract (supernatant) to normalize subsequent enzyme results to the efficiency of the protein extraction.  The enzyme extract is mixed with catechol and Polyphenol Oxidase activity is measured spectrophotometrically by the rate of formation of 1,2 benzoquinone at 410nm.


Information Required by Submitter:

Estimates of activity if available
Composition of sample matrix


Additional Information:

Please keep and ship samples frozen if possible.