Amino Acids - Acid Hydrolyzed Amino Acid Profile

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Price of the test:

$300 per analysis

Acceptable Matrices:

Nearly all raw and processed foods or ingredients.

Limit of Detection:

The detection limit is 0.1%

Sample Size Requirements:

Please provide at least 2g of sample for those products with low protein (<5%). Please provide at least 300mg of sample for those products with high protein (>5%).



Reportable Units:


Turn Around Time:

15 Business Days

Is Rush Available?


Method Reference:

Ion Exchange Chromatography of amino acid profile based on AOAC Official Method 994.12. Internally developed method for sample hydrolysis and extraction.

Method Description:

The peptide linkages in the food sample's proteins are broken by acid hydrolysis. After the acid is removed, the sample is diluted with a buffer solution and filtered. The sample is then injected on an HPLC. Responses are compared to a known calibration standard.

Information Required by Submitter:

An estimate of the total nitrogen or total protein in the sample must be submitted. Medallion Laboratories cannot offer the appropriate test if this information is not available. Alternatively include a request for protein analysis from Medallion Laboratories when submitting your sample.

Additional Information:

This test measures fifteen amino acids, including 8 of the 9 essential amino acids. The  amino acids analyzed include: Alanine, Arginine, Aspartic Acid, Glutamic Acid, Glycine, Histidine, Isoleucine, Leucine, Lysine, Phenylalanine, Proline, Serine, Threonine, Tyrosine, and Valine.

Note: Cysteine, Methionine and Tryptophan are not detected in our analysis.