Folic Acid (Folates) Total IU

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Price of the test:

$114 per analysis

Acceptable Matrices:

This method is applicable to all cereal products, flours and dough products, milk and soy-based products, infant formulas and baby food composites, vegetables, fruits, protein powders and shakes, peanuts and peanut-based products.

Limit of Detection:

5µg/100g - 2,000,000 µg/100g (2 % total folates)

Sample Size Requirements:

10 grams minimum


Ultra-high Performance Liquid Chromatography – MS/MS detection

Reportable Units:


Turn Around Time:

10 Business Days

Is Rush Available?


Method Reference:

AOAC 2011.06

Method Description:

A homogenized sample is weighed and an internal standard and phosphate buffer are added. Amylase and protease enzymes are used to break down the matrix for extraction of the folates. Rat plasma conjugase is used to de-conjugate the polyglutamate forms of folates to mono-glutamates. The extract is cleaned using solid phase extraction (SPE) and the cleaned extract is analyzed by UPLC-MS/MS. Individual vitamers of folates are quantified and the total folates determined and expressed as folic acid. Isotopic folic acid analog (13C-Folic acid) is used as the internal standard.

Additional Information:

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.