Antioxidants in Fats and Oils

PDFdnwIcon Testing Service Catalog (PDF) Print Email


Price of the test:

$175 per analysis

Acceptable Matrices:

Fats and oils in animal and vegetable fats, oils, lards and shortening, as well as antioxidant concentrate.

Unacceptable Matrices:

Food products or ingredients other than listed above.

Limit of Quantitation:

10-2000 ppm

Sample Size Requirements:

20 grams



Reportable Units:


Turn Around Time:

10 Business Days

Is Rush Available?


Method Reference:

AOAC 983.15 and AOCS Ce 6-86

Method Description:

This method detects and quantitates the common phenolic antioxidants: propyl gallate (PG), 2,4,5-trihydroxybutyrophenone (THBP), tert-butylhydroquinone (TBHQ), nordihydroguaiaraetic acid (NDGA), 2-and 3-tert-butyl-4-hydroxyanisole (BHA), 2,6-di-tert-butyl-4-hydroxymethylphenol (Ionox-100), and 2,6-di-tert-butyl-4-hydroxytoluene (BHT). Applicable to animal and vegetable fats, oils, lards, shortenings, as well as antioxidant concentrate dissolved in other solvents. Samples are first dissolved in hexane or 1:1 mixture of acetonitrile: 2-propanol. If the sample is soluble in hexane, the antioxidants are partitioned into acetonitrile, which in turn is concentrated and diluted with 2-propanol (isopropanol). If the sample is soluble in a 1:1 mixture of acetonitrile:2-propanol, the sample is diluted. Antioxidants are then separated and quantitated using reverse-phase gradient elution HPLC using ultraviolet detection at 280 nm.

Information Required by Submitter:

If available, please provide an estimate for the amount of each antioxidant in your sample.

Additional Information:

If present in your sample, the final report will include ppm values for propyl gallate (PG), THBP, TBHQ, NDGA, BHA, and BHT.