Fat by Acid Hydrolysis

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Price of the test:

Acid Hydrolysis (most matrices):$45 per analysis
Combined Acid & Base Hydrolysis (cheese matrices):$45 per analysis
Roese-Gottlieb Method, Alkaline hydrolysis (dairy matrices):$45 per analysis

Acceptable Matrices:

Nearly all food products

Unacceptable Matrices:

This test is not suitable for high-fiber products, i.e. > 25% fiber.

Limit of Detection:


Sample Size Requirements:

10 grams


Standard laboratory equipment

Reportable Units:

% Fat

Turn Around Time:

10 Business Days

Is Rush Available?


Method Reference:

AOAC 922.06, 948.15, 925.32, 950.54, 905.02, 920.111, 945.45G, 920.115F, 932.06, 922.09, 952.06 (Fat in various food stuffs).

Method Description:

Lipids are extracted from food stuffs by acid or alkaline hydrolysis (depending on matrix, hydrolysis type will be matched to matrix) followed by partition into mixed ethers. The extract is then dried and the lipid residue is weighed and expressed as a percent of the original sample weight.

Information Required by Submitter:

For efficiency in processing your sample for analysis, please let your customer service representative know if your sample contains a large percentage of any one particular fatty acid.

Additional Information:

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.

Due to the amount of method variability linked to the sample matrix, we recommend Fat by GC for Nutritional Labeling purposes. Please note that different extraction methods are used for dairy (e.g. milk, ice cream, etc.), egg and cheese products. High fiber content may interfere with this test.