Free Fatty Acids

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Price of the test:

$70 per analysis

Acceptable Matrices:

Fats, oils, flours, nuts, animal foods, mixes

Unacceptable Matrices:

Egg yolks, low fat samples, anything that can’t be extracted with petroleum ether

Limit of Quantitation:

A detection limit of 0.015% can only be achieved with a 56.0 gram sample weight. The detection limit will change based on the sample weight analyzed.

Sample Size Requirements:

Need at least 0.50 grams of fat extracted from food products to run this analysis.


Electronic titrator

Reportable Units:

Samples reported as % FFA as oleic acid.
May also be reported as % lauric acid and % palmitic acid upon request.

Turn Around Time:

10 Business days

Is Rush Available?


Method Reference:

AOCS CA 5a-40

Method Description:

The fat in a sample is extracted into an organic solvent and titrated with sodium hydroxide. Rancidity is calculated as percent oleic acid for most fats and oils. Coconut and palm kernel oils are calculated as percent lauric acid, and palm oil is calculated as percent palmitic acid.

Additional Information:

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.

This test provides a measure of hydrolytic rancidity in an oil or food product. Hydrolytic rancidity involves chemical enzymatic hydrolysis of fats into free fatty acids and glycerol.