Vitamin B12 (Cyanocobalamin)

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Price of the test:

$135 per analysis

Acceptable Matrices:

This method is applicable to cereals, fruit juices, nutritional supplements and meal replacement beverages containing naturally occurring and/or added vitamin B12.

Unacceptable Matrices:

This method is not recommended for use on milk-based infant formulas, and is not designed for use on vitamin concentrates.

Limit of Quantitation:

0.2 µg/100g to 800,000 µg/100g

Sample Size Requirements:

10 grams


Automated pipettor system
Automated plate reader system

Reportable Units:


Turn Around Time:

10 Business Days

Is Rush Available?


Method Reference:

AOAC 952.20 and 986.23

Method Description:

This is a microbiological method employing the organism Lactobacillus delbrueckii to determine vitamin B12 present in food and vitamin concentrates. Vitamin B12 is extracted from a ground, homogenized sample into a solution of sodium phosphate dibasic, citric acid and sodium bisulfite by autoclaving for 10 minutes at 121 - 123 °C. The pH of the solution is adjusted to 6.0 and the diluted extract is added to a basal medium containing all required growth nutrients for L. delbrueckii except vitamin B12. The mixture is inoculated with the microorganism and then incubated for 22 hours at 37°C. The growth response of the microorganism is compared quantitatively to that of known standard solutions of cyanocobalamin (vitamin b12). This method is semi-automated utilizing an automated pipettor system and an automated plate reader system.

Information Required by Submitter:

Please supply an estimate with your sample.

Additional Information:

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.

This method is not designed for use on vitamin concentrates.