Vitamin B6 (Pyridoxine)

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Price of the test:

$104 per analysis

Acceptable Matrices:

This method is applicable to cereals, beverages and dairy products containing added VB6 and/or other free forms or vitamin B6.

Unacceptable Matrices:

This method is not designed for use on vitamin concentrates.

Limit of Quantitation:

0.002 mg/100g to 4000 mg/100 g

Sample Size Requirements:

10 grams


Automated pipettor system
Automated plate reader system

Reportable Units:


Turn Around Time:

10 Business Days

Is Rush Available?


Method Reference:

AOAC 961.15, 985.32, 960.46

Method Description:

This is a microbiological assay employing the organism Saccharomyces uvarum to determine the quantity of Vitamin B6 (pyridoxine, pyridoxal & pyridoxpyridoxine in food products and vitamin concentrates.
The ground, homogenized sample is dissolved or dispersed in an acidic extraction solution. The mixture is autoclaved at 121 - 123 °C to extract vitamin B6. The pH of the extract is adjusted to 4.4 and diluted with a basal medium containing all required growth nutrients for the microorganism except VB6. The mixture is inoculated with the microorganism and then incubated for 22 hours at 30°C. The growth response of the microorganism is compared quantitatively to that of known standard solutions of VB6.
The result is expressed as vitamin B6 (pyridoxine). The method is semi-automated utilizing an Automated Pipettor System and an Automated Plate Reader System.

Information Required by Submitter:

Please supply an estimate with your sample.

Additional Information:

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.

This method is not designed for use on vitamin concentrates.