Niacin (Vitamin B3)

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Price of the test:

$70 per analysis

Acceptable Matrices:

This method is applicable to cereals, beverages and dairy products containing naturally occurring and / or added niacin and niacinamide.

Unacceptable Matrices:

This method is not designed for use on vitamin concentrates.

Limit of Quantitation:

0.007mg / 100g to 20,000mg/100g

Sample Size Requirements:

10 grams


Automated pipettor system
Automated plate reader system

Reportable Units:


Turn Around Time:

10 Business Days

Is Rush Available?


Method Reference:

AOAC 944.13
AOAC 960.46
Microbiological Methods

Method Description:

This is a microbiological method employing the organism Lactobacillus plantarum to determine niacin in food and vitamin concentrates.

The ground, homogenous sample is dissolved in a 1N H2SO4 solution and autoclaved for 30 minutes at 121 - 123 °C. The pH of the solution is adjusted to 6.8. The diluted extract is added to a basal medium containing all required growth nutrients for the microorganism except niacin. The mixture is inoculated with the microorganism and then incubated for 22 hours at 37°C. The growth response of the microorganism is compared quantitatively to that of known standard solutions of niacin. The method is semi-automated utilizing an Automated Pipettor System and an Automated Plate Reader System.

Information Required by Submitter:

Please supply an estimate with your sample.

Additional Information:

This test is ISO/IEC 17025 accredited through A2LA, Certificate # 2769.01.

This method is not designed for use on vitamin concentrates.