Tocopherols and Tocotrienols

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Price of the test:

$175 per analysis

Limit of Detection:

.500 ppm

Sample Size Requirements:

10 grams


HPLC with Fluorescence Detector

Reportable Units:


Turn Around Time:

15 Business Days

Is Rush Available?


Method Reference:

Tocopherol and tocotrienol oils : AACC 86-06
Tocopherol and tocotrienol solids: Internally Developed Method

Method Description:

The fat or food sample is saponified in ethanolic potassium hydroxide. Tocopherol and tocotrienol integrity is protected by adding an antioxidant. Free tocopherols and tocotrienols are extracted from the saponification solution with hexane. The tocopherol and tocotrienol content of the extract is quantitated using normal-phase HPLC with fluorescence detection.

Information Required by Submitter:

Please supply estimates of tocopherol and tocotrienol levels. If this information is not available, please indicate the food type. If this is a concentrated sample, please note this on the analysis request form.

Additional Information:

Tocopherols are very sensitive to oxidation. Be sure the sample is submitted in an airtight container, filled completely if possible. If headspace is present in the container, flushing with nitrogen reduces oxidation. Shipping and handling at reduced temperatures helps maintain the integrity of the sample.