Fiber, Total (Gravimetric)

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Price of the test:

$172 per analysis

Acceptable Matrices:

All foods and food products

Limit of Detection:


Sample Size Requirements:

40 grams


Standard Gravimetric Analytical Chemistry Equipment

Reportable Units:

Percent by weight of sample as received

Turn Around Time:

10 Business Days

Is Rush Available?


Method Reference:

AOAC 991.43 with modifications

Method Description:

Samples (defatted if necessary) are digested with three enzymes (alpha-amylase, protease and amyloglucosidase) to remove starch and protein. Ethanol is added to precipitate soluble dietary fiber. The resulting solution is filtered and the residue is dried and weighed. Protein and ash are determined on the residue. Total dietary fiber (TDF) is calculated as the percent residue minus protein and ash.

Information Required by Submitter:

Please supply expected estimates. Indicate matrix type and indicate high fat (>5%) and high protein (>20%) samples. Indicate whether the sample contains fructans (inulin or fructooligosaccharides (FOS)), or psyllium, flax and gum (i.e. guar gum).

Additional Information:

This test is ISO/IEC 17025 accredited through A2LA, Certificate #2769.01.

Dietary Fiber is the portion of the carbohydrates which is not digested in the human gut. It is composed of both soluble and insoluble components. These methods are applicable to all food products, however samples containing psyllium or fructans should be submitted under specific test codes. High-fat (>5%) and high-protein (>20%, depending on matrix) samples require special handling.